On comparison in the spectra obtained from EI-gC/MS method with spectra collected in nIST library. Such method is suggested as just about the most trusted way of compound identification. The results of this study allow us to present the watersoluble compounds comprised in grasshoppers’ abdominal secretion. The obtained profile of the analyzed material includes 2,108 compounds classified into groups of amino acids, organic acids, carbohydrates, alcohols, hydrocarbons, nucleosides and inorganic acids. Exemplary chromatograms in the analyzed material are presented in the Fig. 1. Compounds Identified in a minimum of 80 of analyzed Samples just after data preprocessing, we chosen 22 compounds identified in all of the analyzed samples obtained from Chorthippus spp. in at least 80 in each and every studied group. The compounds are listed in Table 1. In these groups we could choose: amines, one particular alcohol, a single nucleoside, organic too as inorganic acids and their derivatives, carbohydrates too as amino acids and their derivatives.3-Bromo-5-methylpyrazin-2(1H)-one Chemical name Probably the most abundant group of compounds (9 out of 22 compounds, which constitutes 41 ) was composed of organic acids and their derivatives. The second group of compounds, determined in aqueous fraction of grasshoppers’ abdominal secretion, was composed of amino acids and their derivatives (five out of 22 compounds, 23 ).1,7-Naphthyridin-3-amine Chemical name Compounds and their characteristics, which includes characteristic fragmentation patterns, retention instances and frequency of look in all samples, are presented in Table 1. The mass spectra of 5 exemplary compounds (Uridine, a; Serine, B; Pyrimidine, C; Mannose, D and 5-oxo-Proline, E), identified in 90 of aqueous fractions extracted from grasshoppers abdominal secretions, are presented in Fig. 2a. Figure 2b presents proposed fragmentation pathways of two exemplary compounds, l-Proline- trimethylsilyl ester (a) and N,N-dimethylglycine, trimethylsilyl ester (B).Results and Discussion Folk tales, still well-liked in particular provinces in Poland, carry information about therapeutic properties of many substances. In some of them, grasshopper abdominal secretion was notified to become utilized to stimulate wound healing method. Having said that, there’s no scientific proof till now to rationalize the usage of grasshoppers’ abdominal secretion for healthcare purposes.PMID:35991869 For that reason, we decided to analyze the biochemical composition of grasshopper secretion to identify compounds with probable wound healing potential. It really is known, that compounds obtained from organic solutions can influence many stages of wound healing process. Vitamins (in particular a, E and C), alkaloids, terpenoids,Table 1 Compounds identified in all samples with no less than 80 frequency level tr (min) 26.84 21.77 24.30 24.54 21.14 28.64 17.86 17.42 20.78 20.84 24.15 12.88 24.85 27.06 18.62 15.50 15.77 21.50 26.07 20.16 31.72 36.96 304, 288, 198, 147, 73 160, 117, 58 94 88 84 91 87 92 82 88 90 84 370, 299, 259, 217, 169, 147, 73 90 258, 230, 214, 156, 147, 73, 45 90 216, 170, 142, 73, 45 81 261, 218, 158, 73, 45 91 221, 190, 73, 59, 45, 29 204, 176, 147, 102, 73, 45 83 92 319, 217, 205, 160, 147, 129, 127 97 Carbohydrate Organic acid amino acid derivatives amino acid derivatives amino acid derivatives amino acid derivatives amino acid derivatives Organic acid derivatives Inorganic acid derivatives Inorganic acid derivatives Organic acid derivatives Organic acid derivatives Organic derivatives Carbohydrate amino acid derivatives Organic acid derivatives nucleoside 90 Organic a.
